PROJECT TITLE :

Extension and denature temperature of dsDNA molecules at various magnetic forces by using magnetic tweezers

ABSTRACT:

Stretching experiments on lambda-deoxyribonucleic acid (DNA) were carried out in microfluidic channel employing a microscope, micro-flow pump, and electromagnet. The micro-flow channel was fabricated by using micro electromechanical technology, and also the electromagnet was analyzed by software to simulate the magnetic field distribution. The magnetic field was 248.six gauss at a current of zero.five A, and therefore the system may exert twenty.four pN of force on a magnetic bead with diameter of two.8 μm. When the buffer temperature was twenty five °C, the average length of lambda-DNA molecules was 1.9 μm while not magnetic field. During a magnetic field of 248.half-dozen gauss, the extension was 7.a pair of μm, the entire length was 9.1 μm, and the coefficient of elasticity was two.eighty three × ten-vi N/m. When the buffer temperature was forty five °C, the common length was 4.vi μm while not magnetic field. At forty five °C in a magnetic field of 248.half-dozen gauss, the extension was 11.9 μm, the whole length was 16.5 μm, and also the coefficient of elasticity was 1.71 × ten-half-dozen N/m. Besides, the denature temperature of dsDNA decreases with increasing magnetic force, and the temperature distinction will be on top of seven °C. This system can be used to measure the related properties of macromolecules.


Did you like this research project?

To get this research project Guidelines, Training and Code... Click Here


PROJECT TITLE : PEVA: A Page Endurance Variance Aware Strategy for the Lifetime Extension of NAND Flash - 2016 ABSTRACT: With aggressive scaling and multilevel cell technology, the reliability of NAND flash continuously degrades.
PROJECT TITLE :A Novel Humidity Sensor Based on the Extension of Thompson and Lampard TheoremABSTRACT:This paper deals with the event of an correct and precise humidity sensor, that is predicated on the extension of Thompson and
PROJECT TITLE :Validation and Extension of Local Temperature Evaluation of Conductive Filaments in RRAM DevicesABSTRACT:Native temperature plays a key role in resistive switching random access memory devices. We tend to have previously
PROJECT TITLE :A Study on Energy Saving and $hbox_ $ Emission Reduction on Signal Countdown Extension by Vehicular Ad Hoc NetworksABSTRACT:Research on the broadcasting of signal countdown messages (SCMs) to vehicles via vehicular
PROJECT TITLE :Extension of the Generalized Split-Window Algorithm for Land Surface Temperature Retrieval to Atmospheres With Heavy Dust Aerosol LoadingABSTRACT:It's price noting that the influences of dust aerosol type and totally

Ready to Complete Your Academic MTech Project Work In Affordable Price ?

Project Enquiry