Extension and denature temperature of dsDNA molecules at various magnetic forces by using magnetic tweezers


Stretching experiments on lambda-deoxyribonucleic acid (DNA) were carried out in microfluidic channel employing a microscope, micro-flow pump, and electromagnet. The micro-flow channel was fabricated by using micro electromechanical technology, and also the electromagnet was analyzed by software to simulate the magnetic field distribution. The magnetic field was 248.six gauss at a current of zero.five A, and therefore the system may exert twenty.four pN of force on a magnetic bead with diameter of two.8 μm. When the buffer temperature was twenty five °C, the average length of lambda-DNA molecules was 1.9 μm while not magnetic field. During a magnetic field of 248.half-dozen gauss, the extension was 7.a pair of μm, the entire length was 9.1 μm, and the coefficient of elasticity was two.eighty three × ten-vi N/m. When the buffer temperature was forty five °C, the common length was μm while not magnetic field. At forty five °C in a magnetic field of 248.half-dozen gauss, the extension was 11.9 μm, the whole length was 16.5 μm, and also the coefficient of elasticity was 1.71 × ten-half-dozen N/m. Besides, the denature temperature of dsDNA decreases with increasing magnetic force, and the temperature distinction will be on top of seven °C. This system can be used to measure the related properties of macromolecules.

Did you like this research project?

To get this research project Guidelines, Training and Code... Click Here

PROJECT TITLE : PEVA: A Page Endurance Variance Aware Strategy for the Lifetime Extension of NAND Flash - 2016 ABSTRACT: With aggressive scaling and multilevel cell technology, the reliability of NAND flash continuously degrades.
PROJECT TITLE :A Study on Energy Saving and $hbox_ $ Emission Reduction on Signal Countdown Extension by Vehicular Ad Hoc NetworksABSTRACT:Research on the broadcasting of signal countdown messages (SCMs) to vehicles via vehicular
PROJECT TITLE :Fractional order Sprott chaos synchronisation-based real-time extension power quality detection methodABSTRACT:It is known that real-time power quality monitoring is typically rather troublesome to realize due to
PROJECT TITLE : Video Dissemination over Hybrid Cellular and Ad Hoc Networks - 2014 ABSTRACT: We study the problem of disseminating videos to mobile users by using a hybrid cellular and ad hoc network. In particular, we formulate
PROJECT TITLE : Secure and Efficient Data Transmission for Cluster-Based Wireless Sensor Networks - 2014 ABSTRACT: Secure data transmission is a critical issue for wireless sensor networks (WSNs). Clustering is an effective

Ready to Complete Your Academic MTech Project Work In Affordable Price ?

Project Enquiry