PROJECT TITLE :

Remote Axial Tuning in Microscopy Utilizing Hydrogel-Driven Tunable Liquid Lens

ABSTRACT:

This paper presents the employment of a tunable-focus thermoresponsive hydrogel-based mostly liquid lens together with an objective lens to realize remote axial focusing in conventional microscopy. The goal of this style is to eliminate image distortion because of sample vibrations caused by mechanical stage scanning. This approach reduces the mechanical complexity and power consumption because of the use of electrically tunable lenses, whereas achieving a twofold increase in the axial scanning vary. The deserves of the proposed style were demonstrated by characterizing a customized microscope system over a scanning range of 1700 μm. A lateral resolution of two μm was obtained consistently throughout the scanning range. Healthy Spodopterafrugiperda Sf21 insect cells imaging was used to verify the depth scanning ability and also the resolution of our remote focusing microscope system.