An Integrated Circuit for Chip-Based Analysis of Enzyme Kinetics and Metabolite Quantification


We have created a completely unique chip-based diagnostic tools primarily based upon quantification of metabolites using enzymes specific for his or her chemical conversion. Using this device we tend to show for the first time that a solid-state circuit can be used to measure enzyme kinetics and calculate the Michaelis-Menten constant. Substrate concentration dependency of enzyme reaction rates is central to this aim. Ion-sensitive field effect transistors (ISFET) are excellent transducers for biosensing applications that are reliant upon enzyme assays, especially since they'll be fabricated using mainstream microelectronics technology to confirm low unit value, mass-manufacture, scaling to make several sensors and simple miniaturisation for use in point-of-care devices. Here, we have a tendency to describe an integrated ISFET array comprising 216 sensors. The device was fabricated with a complementary metal oxide semiconductor (CMOS) process. Not like traditional CMOS ISFET sensors that use the Si3N4 passivation of the foundry for ion detection, the device reported here was processed with a layer of Ta2O5 that increased the detection sensitivity to forty five mV/pH unit at the sensor readout. The drift was reduced to zero.eight mV/hour with a linear pH response between pH 2-12. A high-speed instrumentation system capable of acquiring nearly five hundred fps was developed to stream out the information. The device was then used to measure glucose concentration through the activity of hexokinase within the vary of 0.05 mM-231 mM, encompassing glucose's physiological vary in blood. Localised and temporal enzyme kinetics of hexokinase was studied in detail. These results present a roadmap towards a viable personal metabolome machine.

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