PROJECT TITLE :

Synthesis of silver nanoparticles by Bacillus subtilis T-1 growing on agro-industrial wastes and producing biosurfactant

ABSTRACT:

During this study, culture supernatnats of Bacillus subtilis T-1 growing on brewery effluents and molasses was used for silver nanoparticles (Ag-NPs) synthesis. The biosurfactant production of B. subtilis T-one was confirmed by the detection of genes within the genome and by the identification of the merchandise within the supernatants. The genes for synthesis of surfactin (sfp, srfAA) and iturin (ituC) were noted by PCR reactions. Also, in examined culture supernatants the presence of C13, C14 and C15 surfactin homologues with the sodiated molecules [M + Na]+ at m/z 1030, 104four and 1058 was confirmed using LC/MS/MS analysis. The formation of NPs in the culture supernatants was confirmed by UV–vis spectroscopy. The dynamic light-weight scattering measurements and transmission electron microscopy images showed the nanometric sizes of the biosynthesised Ag-NPs that ranged from many nm to many tens of nm depending on the used culture supernatant. Biological properties of Ag-NPs were evaluated by binding of Ag-NPs with DNA isolated from the Escherichia coli ATCC 2592two and B. subtilis ATCC 6633. Biogenic Ag-NPs were actively certain to DNA in increased concentration which may be the one necessary mode of antibacterial action of the Ag-NPs.