PROJECT TITLE :

Biosensing Application of Hybrid Thin-Film Layers-Based Biosensors

ABSTRACT:

Biosensor based mostly on immobilized tyrosinase on Langmuir-Blodgett skinny film of arachidic acid and dysprosium bis-phthalocyanine has been developed. The biocomposite skinny film has been characterised by UV-Vis spectroscopy, Fourier transformed infrared absorption spectroscopy, atomic force microscopy, and electrochemistry. Enzyme immobilization has been proved, and it maintain well the biocatalytic properties onto solid biocomposite film. The electrochemical detection of biosensor has been performed by measuring the cathodic current thanks to the reduction of the corresponding quinone at zero.024 V. Beneath optimum conditions, the performance characteristics of biosensors have been obtained. A linear response range from five up to seventy five μM of dopamine has been obtained. The detection limit is 7.eleven × ten-7 M for developed biosensors. The biosensor construction is highly reproducible because of use of Langmuir-Blodgett nanotechnology. Finally, the developed biosensor has been applied to the determination of dopamine content in pharmaceutical samples.