PROJECT TITLE :

A High-Throughput Automated Microinjection System for Human Cells With Small Size

ABSTRACT:

This paper presents the event of an automated microinjection system with high productivity for tiny cells. Compared with several existing microinjection systems that are primarily designed for relatively massive-scaled biological samples, the reported technology can enable an automated injection into the cells with a diameter smaller than twenty five μm, that is the standard size of the many human cells. Microfluidic technology has been used to design a vacuum-based mostly cell-holding device for immobilizing cells. The cell holder employs tons of standard-formed channels, and every channel is occupied by one cell. The vision-based mostly position-tracking method is employed to recognize and position the target cells automatically, in which the cell position is measured by edge template-matching methodology, and a bright field image is employed to simplify the popularity process. A three-DOF microrobotic system mounted with a micropipette is employed to conduct cell injection task with the speed of 35 cells/min and also the precision of zero.two μm. Injection experiments on human foreskin fibroblast and human embryonic stem cell-derived ventricular cardiomyocyte were performed to demonstrate the effectiveness of the developed system. Additional than a thousand cells were injected with fluorescent markers. Experimental results showed that the developed injection system exhibits high productivity and accuracy, and leads to a good cell survival rate once injection.