Extension and denature temperature of dsDNA molecules at various magnetic forces by using magnetic tweezers PROJECT TITLE :Extension and denature temperature of dsDNA molecules at various magnetic forces by using magnetic tweezersABSTRACT:Stretching experiments on lambda-deoxyribonucleic acid (DNA) were carried out in microfluidic channel employing a microscope, micro-flow pump, and electromagnet. The micro-flow channel was fabricated by using micro electromechanical technology, and also the electromagnet was analyzed by software to simulate the magnetic field distribution. The magnetic field was 248.six gauss at a current of zero.five A, and therefore the system may exert twenty.four pN of force on a magnetic bead with diameter of two.8 μm. When the buffer temperature was twenty five °C, the average length of lambda-DNA molecules was 1.9 μm while not magnetic field. During a magnetic field of 248.half-dozen gauss, the extension was 7.a pair of μm, the entire length was 9.1 μm, and the coefficient of elasticity was two.eighty three × ten-vi N/m. When the buffer temperature was forty five °C, the common length was 4.vi μm while not magnetic field. At forty five °C in a magnetic field of 248.half-dozen gauss, the extension was 11.9 μm, the whole length was 16.5 μm, and also the coefficient of elasticity was 1.71 × ten-half-dozen N/m. Besides, the denature temperature of dsDNA decreases with increasing magnetic force, and the temperature distinction will be on top of seven °C. This system can be used to measure the related properties of macromolecules. Did you like this research project? To get this research project Guidelines, Training and Code... Click Here facebook twitter google+ linkedin stumble pinterest Fluorescence microscopy investigation of InGaN-based light-emitting diodes A Review of Algorithms for Compliant Control of Stiff and Fixed-Compliance Robots