PROJECT TITLE :
In vitro anti-foot-and-mouth disease virus activity of magnesium oxide nanoparticles
Foot-and-mouth disease (FMD) is an extraordinarily contagious viral disease of cloven-hoofed animals which will lead to very large economic losses in the livestock production. No antiviral therapies are available for treating FMD virus (FMDV) infections in animals. The antiviral effects of magnesium oxide nanoparticles (MgO NPs) on the FMDV were investigated in cell culture. The viability of the cells when MgO NP treatment determined using the MTT [3-(4,5-dimethylthiazol-a pair of-yl)-two,5-diphenyltetrazolium bromide] assay. The direct effects of MgO NPs on the FMDV in extracellular (virucidal assay) and also completely different stages of virus replication (antiviral assay) were evaluated by plaque reduction assay. The results showed that MgO NPs were safe at concentrations up to 250 μg/ml within the Razi Bovine kidney cell line. The treatments with NPs indicated that the MgO NPs exerted in vitro virucidal and antiviral activities. Plaque reduction assay revealed that MgO NPs can inhibit FMDV by additional than ninetypercent at the early stages of infection such as attachment and penetration but not after penetration. The results of this study steered that NPs would possibly be applied regionally as an antiviral agent in early stages of infection in inclined animals.
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