PROJECT TITLE :

Synthetic Biology: A Unifying View and Review Using Analog Circuits

ABSTRACT:

We have a tendency to review the sphere of artificial biology from an analog circuits and analog computation perspective, focusing on circuits that are designed in living cells. This perspective is similar temperament to pictorially, symbolically, and quantitatively representing the nonlinear, dynamic, and stochastic (noisy) ordinary and partial differential equations that rigorously describe the molecular circuits of artificial biology. This perspective permits us to construct a canonical analog circuit schematic that helps unify and review the operation of the many fundamental circuits that are built in synthetic biology at the DNA, RNA, protein, and tiny-molecule levels over nearly two decades. We have a tendency to review 17 circuits within the literature as explicit samples of feedforward and feedback analog circuits that arise from special topological cases of the canonical analog circuit schematic. Digital circuit operation of these circuits represents a special case of saturated analog circuit behavior and is automatically incorporated additionally. Several problems that have prevented artificial biology from scaling are naturally represented in analog circuit schematics. Furthermore, the deep similarity between the Boltzmann thermodynamic equations that describe noisy electronic current flow in subthreshold transistors and noisy molecular flux in biochemical reactions has helped map analog circuit motifs in electronics to analog circuit motifs in cells and vice versa via a 'cytomorphic' approach. Thus, a body of information in analog electronic circuit style, analysis, simulation, and implementation may conjointly be helpful within the robust and economical design of molecular circuits in synthetic biology, serving to it to scale to more complicated circuits in the long run.


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