Influenza A virus (FLUAV), the causative agent of influenza infection, has received extensive attention due to the recent swine-origin H1N1 pandemic. FLUAV has long been the cause of annual epidemics as well as less frequent but more severe global pandemics. Here, we describe a biosensor utilizing electrically active magnetic (EAM) polyaniline-coated nanoparticles as the transducer in an electrochemical biosensor for rapidly identifying FLUAV strains based on receptor specificity, which will be useful to monitor animal influenza infections and to characterize pandemic potential of strains that have transmitted from animals to humans. Pandemic potential requires human-to-human transmissibility, which is dependent upon FLUAV hemagglutinin (HA) specificity for host glycan receptors. Avian FLUAV preferentially bind to α2,3-linked receptors, while human FLUAV bind to α2,6-linked receptors. EAM nanoparticles were prepared by synthesizing aniline monomer around gamma iron (III) oxide (γ-Fe2O3) cores, yielding 25-100-nm diameter nanoparticles that were structurally characterized by transmission electron microscopy and electron diffraction. The EAM nanoparticles were coated with monoclonal antibodies specific to H5N1 (A/Vietnam/1203/04). Specificity of binding between glycans and H5 was demonstrated. The biosensor results were correlative to supporting data from a surface plasmon resonance assay that characterized HA/glycan binding and α-H5 antibody activity. This novel study applies EAM nanoparticles as the transducer in a specific, portable, easy-to-use biosensor with great potential for disease monitoring and biosecurity applications.

Did you like this research project?

To get this research project Guidelines, Training and Code... Click Here

PROJECT TITLE :Computational Study of Interdependence Between Hemagglutinin and Neuraminidase of Pandemic 2009 H1N1ABSTRACT:Influenza sort A viruses are classified into subtypes based on their two surface proteins, hemagglutinin
PROJECT TITLE :Modeling, Detection, and Diagnosis of Faults in Multilevel Memristor MemoriesABSTRACT:Memristors are an attractive choice for use in future memory architectures but are vulnerable to high defect densities thanks
PROJECT TITLE:An Unified Multiscale Framework for Planar, Surface, and Curve SkeletonizationABSTRACT:Computing skeletons of 2D shapes, and medial surface and curve skeletons of 3D shapes, could be a difficult task. In particular,
PROJECT TITLE : Network Resource Allocation for Users With Multiple Connections Fairness and Stability - 2014 ABSTRACT: This paper studies network resource allocation between users that manage multiple connections, possibly
PROJECT TITLE :Distance Bounding A Practical Security Solution for Real-Time Location Systems - 2013ABSTRACT:The need for implementing adequate security services in industrial applications is increasing. Verifying the physical

Ready to Complete Your Academic MTech Project Work In Affordable Price ?

Project Enquiry