Enzyme Sensor With Polydimethylsiloxane Membrane and CMOS Potentiostat for Wide-Range Glucose Measurements


We tend to report on an electrochemical measurement setup comprising a glucose sensor and a CMOS potentiostat with a two-layer membrane as the primary steps toward the event of an integrated in-situ sensor system for bioreactors. The potentiostat includes a chip size of two.1 mm × two.5 mm and a linear current vary from -220 nA to 240 nA with a linearity of R2 = zero.9995. For big selection measurements of glucose concentrations in cell culture media, electrodes functionalized with the enzyme glucose oxidase were spin-coated with membranes created from polydimethylsiloxane (PDMS). A two-stage curing theme of the PDMS was applied, and totally different membrane thicknesses and curing times were evaluated. With these membranes, glucose concentrations up to five hundred mM were measured with a linear measurement vary up to 200 mM. The sensors were successfully used in the glucose monitoring of a culture of Saccharomyces cerevisiae to watch the glucose consumption of the cells. For interference elimination, the cellulose acetate membranes were used.

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